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Proteomic Analysis of Pancreatic Cyst Fluids

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Proteomic Analysis of Pancreatic Cyst Fluids
There are currently no diagnostic indicators that are consistently reliable, obtainable, and conclusive for diagnosing and risk-stratifying pancreatic cysts. To establish more effective diagnostic biomarkers and to provide deeper understanding about the molecular profile within these cysts, we identified and quantified approximately 500 cyst fluid proteins and correlated the findings to clinical parameters. Pancreatic cyst fluids were collected by endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) from 20 patients. The proteins in the cyst fluids were ascertained by LC/MS/MS analysis on a highly accurate and stable mass spectrometer. From just 15 micrograms of protein from less than 40 microliters of cyst fluid per sample, we simultaneously measured traditional pancreatic cancer markers of mucins, amylase, and CEA. The ease of measuring soluble mucins by MS sensitively and specifically is a significant progress. We further propose to use of a panel of homologs of these biomarkers, namely, two homologs of amylase, five soluble mucins, five soluble CEA-related cell adhesion molecules (CEACAMs), and four S100 homologs, to facilitate future pancreatic cyst diagnosis and risk-stratification.

This manuscript of the findings is in press to appear the week of March 1 2009 in the journal Pancreas.
All supplemental data will appear in this site after publication.

Created by: yeung Last Modification: Tuesday 16 of December, 2008 22:35:45 EST by yeung